LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2459..2555) /label=attL2 /note="attL2 (c)" misc_signal complement(2570..2589) /note="T7 Promoter/priming site (c)" primer_bind 2597..2613 /note="M13 Reverse priming site" gene 2726..3535 /note="Kanamycin resistance gene" rep_origin 3656..4329 /note="pUC origin" vector join(2467..4332,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2452 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:8691 IMAGE:2964645" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..2452 /gene="OS9" /gene_synonym=OS-9 /db_xref="GeneID:10956" /db_xref="HGNC:16994" /db_xref="MIM:609677" CDS 692..2452 /dnas_title="osteosarcoma amplified 9, endoplasmic reticulum associated protein" /gene="OS9" /gene_synonym=OS-9 /codon_start=1 /product="osteosarcoma amplified 9, endoplasmic reticulum associated protein" /protein_id="AAH00532.1" /db_xref="GI:12653521" /db_xref="GeneID:10956" /db_xref="HGNC:16994" /db_xref="MIM:609677" /translation="MAAETLLSSLLGLLLLGLLLPASLTGGVGSLNLEELSEMRYGIE ILPLPVMGGQSQSSDVVIVSSKYKQRYECRLPAGAIHFQREREEETPAYQGPGIPELL SPMRDAPCLLKTKDWWTYEFCYGRHIQQYHMEDSEIKGEVLYLGYYQSAFDWDDETAK ASKQHRLKRYHSQTYGNGSKCDLNGRPREAEVRFLCDEGAGISGDYIDRVDEPLSCSY VLTIRTPRLCPHPLLRPPPSAAPQAILCHPSLQPEEYMAYVQRQADSKQYGDKIIEEL QDLGPQVWSETKSGVAPQKMAGASPTKDDSKDSDFWKMLNEPEDQAPGGEEVPAEEQD PSPEAADSASGAPNDFQNNVQVKVIRSPADLIRFIEELKGGTKKGKPNIGQEQPVDDA AEVPQREPEKERGDPERQREMEEEEDEDEDEDEDEDERQLLGEFEKELEGILLPSDRD RLRSEVKAGMERELENIIQETEKELDPDGLKKESERDRAMLALTSTLNKLIKRLEEKQ SPELVKKHKKKRVVPKKPPPSPQPTGKIEIKIVRPWAEGTEEGARWLTDEDTRNLKEI FFNILVPGAEEAQKERQRQKELESNYRRVWGSPGGEGTGDLDEFDF" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cggcggtgtc gggagcctga acctggagga 721 gctgagtgag atgcgttatg ggatcgagat cctgccgttg cctgtcatgg gagggcagag 781 ccaatcttcg gacgtggtga ttgtctcctc taagtacaaa cagcgctatg agtgtcgcct 841 gccagctgga gctattcact tccagcgtga aagggaggag gaaacacctg cttaccaagg 901 gcctgggatc cctgagttgt tgagcccaat gagagatgct ccctgcttgc tgaagacaaa 961 ggactggtgg acatatgaat tctgttatgg acgccacatc cagcaatacc acatggaaga 1021 ttcagagatc aaaggtgaag tcctctatct cggctactac caatcagcct tcgactggga 1081 tgatgaaaca gccaaggcct ccaagcagca tcgtcttaaa cgctaccaca gccagaccta 1141 tggcaatggg tccaagtgcg accttaatgg gaggccccgg gaggccgagg ttcggttcct 1201 ctgtgacgag ggtgcaggta tctctgggga ctacatcgat cgcgtggacg agcccttgtc 1261 ctgctcttat gtgctgacca ttcgcactcc tcggctctgc ccccaccctc tcctccggcc 1321 cccacccagt gctgcaccgc aggccatcct ctgtcaccct tccctacagc ctgaggagta 1381 catggcctac gttcagaggc aagccgactc aaagcagtat ggagataaaa tcatagagga 1441 gctgcaagat ctaggccccc aagtgtggag tgagaccaag tctggggtgg caccccaaaa 1501 gatggcaggt gcgagcccga ccaaggatga cagtaaggac tcagatttct ggaagatgct 1561 taatgagcca gaggaccagg ccccaggagg ggaggaggtg ccggctgagg agcaggaccc 1621 aagccctgag gcagcagatt cagcttctgg tgctcccaat gattttcaga acaacgtgca 1681 ggtcaaagtc attcgaagcc ctgcggattt gattcgattc atagaggagc tgaaaggtgg 1741 aacaaaaaag gggaagccaa atataggcca agagcagcct gtggatgatg ctgcagaagt 1801 ccctcagagg gaaccagaga aggaaagggg tgatccagaa cggcagagag agatggaaga 1861 agaggaggat gaggatgagg atgaggatga agatgaggat gaacggcagt tactgggaga 1921 atttgagaag gaactggaag ggatcctgct tccgtcagac cgagaccggc tccgttcgga 1981 ggtgaaggct ggcatggagc gggaactgga aaacatcatc caggagacag agaaagagct 2041 ggacccagat gggctgaaga aggagtcaga gcgggatcgg gcaatgctgg ctctcacatc 2101 cactctcaac aaactcatca aaagactgga ggaaaaacag agtccagagc tggtgaagaa 2161 gcacaagaaa aagagggttg tccccaaaaa gcctccccca tcaccccaac ctacagggaa 2221 aattgagatc aaaattgtcc gcccatgggc tgaagggact gaagagggtg cacgttggct 2281 gactgatgag gacacgagaa acctcaagga gatcttcttc aatatcttgg tgccgggagc 2341 tgaagaggcc cagaaggaac gccagcggca gaaagagctg gagagcaatt accgccgggt 2401 gtggggctct ccaggtgggg agggcacagg ggacctggac gaatttgact tcTAGGACCC 2461 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA 2521 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG 2581 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT 2641 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA 2701 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG 2761 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG 2821 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT 2881 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG 2941 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC 3001 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC 3061 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT 3121 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC 3181 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC 3241 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT 3301 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG 3361 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA 3421 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA 3481 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA 3541 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG 3601 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT 3661 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 3721 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 3781 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA 3841 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 3901 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 3961 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 4021 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 4081 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 4141 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 4201 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 4261 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 4321 TGCTCACATG TT //